Sammanfattning

BackgroundThe outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCR-genotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.ResultsWe have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd's Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.ConclusionsThis novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.

Nyckelord

Outcrossing; Mixed mating; Outcrossing rate; Capsella; Amplicon sequencing

Publicerad i

Plant Methods
2019, Volym: 15, artikelnummer: 47
Utgivare: BMC

UKÄ forskningsämne

Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1186/s13007-019-0433-9

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/100341