Sammanfattning

Environmental concentrations of the anxiolytic drug oxazepam have been found to disrupt antipredator behaviors of wild fish. Most experiments exposed fish for a week, while evidence from mammals suggests that chronic exposure to therapeutic concentrations of benzodiazepines (such as oxazepam) results in the development of tolerance to the anxiolytic effects. If tolerance can also develop in response to the low concentrations found in the aquatic environment, it could mitigate the negative effects of oxazepam pollution. In the current study, we exposed wild-caught zebrafish to oxazepam (similar to 7 mu g L-1) for 7 or 28 days and evaluated behavioral and physiological parameters at both time points. Females showed reduced diving responses to conspecific alarm pheromone after 7 days, but not after 28 days, indicating that they had developed tolerance to the anxiolytic effects of the drug. Zebrafish males were not affected by this oxazepam concentration, in line with earlier results. Serotonin turnover (ratio 5-HIAA/5-HT) was reduced in exposed females and males after 28 days, indicating that brain neurochemistry had not normalized. Post-confinement cortisol concentrations and gene expression of corticotropin-releasing hormone (CRH) were not affected by oxazepam. We did not find evidence that chronically exposed fish had altered relative expression of GABA A receptor subunits, suggesting that some other still unknown mechanism caused the developed tolerance.

Publicerad i

Environmental Science and Technology
2020, Volym: 54, nummer: 3, sidor: 1760-1769
Utgivare: AMER CHEMICAL SOC

SLU författare

• Brodin, Tomas

  • Institutionen för vilt, fisk och miljö, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Zoologi
Fisk- och akvakulturforskning


Publikationens identifierare

DOI: https://doi.org/10.1021/acs.est.9b06052

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/104716