Sammanfattning

Vegetative propagation relies on the capacity of plants to regeneratede novoadventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. Here, we explored the role of red light (RL) on ARI in de-rooted Norway spruce seedlings. We combined investigation of hormone metabolism and gene expression analysis to identify potential signaling pathways. We also performed extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins. We demonstrated that exogenously applied JA and/or CK inhibit ARI in a dose-dependent manner and found that they possibly act in the same pathway. The negative effect of JA on ARI was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.

Nyckelord

adventitious roots; conifers; Picea abies; auxin; cytokinins; jasmonate; red light; root development

Publicerad i

Frontiers in Plant Science
2020, Volym: 11, artikelnummer: 586140
Utgivare: FRONTIERS MEDIA SA

SLU författare

• Novak, Ondrej

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    
  • Institute of Experimental Botany ASCR
  • Univerzity Palackého v Olomouci

UKÄ forskningsämne

Botanik


Publikationens identifierare

DOI: https://doi.org/10.3389/fpls.2020.586140

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/108219