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Forskningsartikel2020Vetenskapligt granskadÖppen tillgång

Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model Systems

Arora, Deepanksha; Abel, Nikolaj B.; Liu, Chen; Van Damme, Petra; Yperman, Klaas; Eeckhout, Dominique; Vu, Lam Dai; Wang, Jie; Tornkvist, Anna; Impens, Francis; Korbei, Barbara; Van Leene, Jelle; Goossens, Alain; De Jaeger, Geert; Ott, Thomas; Moschou, Panagiotis Nikolaou; Van Damme, Daniel

Sammanfattning

Proximity labeling is a powerful approach for detecting protein-protein interactions. Most proximity labeling techniques use a promiscuous biotin ligase or a peroxidase fused to a protein of interest, enabling the covalent biotin labeling of proteins and subsequent capture and identification of interacting and neighboring proteins without the need for the protein complex to remain intact. To date, only a few studies have reported on the use of proximity labeling in plants. Here, we present the results of a systematic study applying a variety of biotin-based proximity labeling approaches in several plant systems using various conditions and bait proteins. We show that TurboID is the most promiscuous variant in several plant model systems and establish protocols that combine mass spectrometry-based analysis with harsh extraction and washing conditions. We demonstrate the applicability of TurboID in capturing membrane-associated protein interactomes using Lotus japonicus symbiotically active receptor kinases as a test case. We further benchmark the efficiency of various promiscuous biotin ligases in comparison with one-step affinity purification approaches. We identified both known and novel interactors of the endocytic TPLATE complex. We furthermore present a straightforward strategy to identify both nonbiotinylated and biotinylated peptides in a single experimental setup. Finally, we provide initial evidence that our approach has the potential to suggest structural information of protein complexes.A systematic study applying a variety of biotin-based proximity labeling approaches in several plant systems using various conditions and bait proteins.

Publicerad i

Plant Cell
2020, Volym: 32, nummer: 11, sidor: 3388-3407
Utgivare: AMER SOC PLANT BIOLOGISTS

      SLU författare

        • UKÄ forskningsämne

        Cellbiologi
        Växtbioteknologi

        More information

        Correction in: The Plant Cell, 2022, Volume 34, Issue 7, DOI: 10.1093/plcell/koac101

        Publikationens identifierare

        DOI: https://doi.org/10.1105/tpc.20.00235

        Permanent länk till denna sida (URI)

        https://res.slu.se/id/publ/110117