Sammanfattning

Cyanovirin-N (CV-N) is a cyanobacterial lectin with antiviral activity towards HIV and several other viruses. Here, we identify mannoside hydroxyl protons that are hydrogen bonded to the protein backbone of the CV-N domain B binding site, using NMR spectroscopy. For the two carbohydrate ligands Manα(1→2)ManαOMe and Manα(1→2) Manα(1→6)ManαOMe five hydroxyl protons are involved in hydrogen-bonding networks. Comparison with previous crystallographic results revealed that four of these hydroxyl protons donate hydrogen bonds to protein backbone carbonyl oxygens in solution and in the crystal. Hydrogen bonds were not detected between the side chains of Glu41 and Arg76 with sugar hydroxyls, as previously proposed for CV-N binding of mannosides. Molecular dynamics simulations of the CV-N/Manα(1→2)Manα(1→6)ManαOMe complex confirmed the NMR-determined hydrogen-bonding network. Detailed characterization of CV-N/mannoside complexes provides a better understanding of lectin-carbohydrate interactions and opens up to the use of CV-N and similar lectins as antiviral agents.

Nyckelord

carbohydrates; cyanovirin-N; hydrogen bonds; hydroxyls; NMR spectroscopy

Publicerad i

Glycobiology
2021, Volym: 31, nummer: 4, sidor: 508-518

SLU författare

• Nestor, Gustav

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    
  • University of Pittsburgh

UKÄ forskningsämne

Strukturbiologi


Publikationens identifierare

DOI: https://doi.org/10.1093/glycob/cwaa081

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/112183