Sammanfattning

The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m(3)) continuously operated fermenters to replace 10% of the fish feed protein used in Norway.

Nyckelord

Protein hydrolysate; Single-cell protein; Aquaculture; Downstream processing; Repeated batch

Publicerad i

Bioprocess and Biosystems Engineering
2020, Volym: 43, nummer: 4, sidor: 723-736
Utgivare: SPRINGER

SLU författare

• Passoth, Volkmar

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Mikrobiologi


Publikationens identifierare

DOI: https://doi.org/10.1007/s00449-019-02271-x

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/112387