Sammanfattning

Microbial mineralization is increasingly used in bioremediation of heavy metal pollution, but better mechanistic understanding of the processes involved and how they are regulated are required to improve the practical application of microorganisms in bioremediation. We used a combination of morphological (TEM) and analytical (XRD, XPS, FTIR) methods, together with novel proteomic analyses, to investigate the detoxification mechanisms, used by a range of bacteria, including the strains Bacillus velezensis LB002, Escherichia coli DH5α, B. subtilis 168, Pseudomonas putida KT2440, and B. licheniformis MT-1, exposed to elevated concentrations of Cd2+ and combinations of Cd2+, Pb2+, Cu2+, and Zn2+, in the presence and absence of added CaCl2. Common features of detoxification included biomineralization, including the production of biological vaterite, up-regulation of proteins involved in flagellar movement and chemotaxis, biofilm synthesis, transmembrane transport of small molecules and organic matter decomposition. The putative roles of differentially expressed proteins in detoxification are discussed in relation to chemical and morphological data and together provide important tools to improve screening, selection, and practical application of bacterial isolates in bioremediation of polluted environments.

Nyckelord

Metallogenic detoxification; Heavy metal; Microbial remediation; Proteomics; Molecular mechanism

Publicerad i

Ecotoxicology and Environmental Safety
2022, Volym: 246, artikelnummer: 114193

SLU författare

• Finlay, Roger

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Annan geovetenskap och miljövetenskap
Mikrobiologi
Miljövetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.ecoenv.2022.114193

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/119321