Sammanfattning

The storage of boar semen samples at 17 degrees C for artificial insemination (AI) doses enables the proliferation of the bacteria, making antibiotics necessary. This can contribute to the development of antimicrobial resistance (AMR). This study tested bacterial presence and sperm chromatin structure after using a low-density colloid (Porcicoll) as an antibiotic alternative to eliminate bacteria. Ejaculates (8 boars, 3 ejac-ulates each) were split as control and low-density colloid centrifugation (single layer centrifugation, SLC, 20%, and 30% Porcicoll) into 500 ml tubes. Analyses were carried out at days 0, 3, and 7 (17 degrees C) for microbial presence and sperm chromatin structure analysis: %DFI (DNA fragmentation) and %HDS (chromatin immaturity), monobromobimane (mBBr; free thiols and disulfide bridges), and chromomycin A3 (CMA3; chromatin compaction). Besides comparing bacterial presence (7 species identified) and chromatin variables between treatments, the associations between these sets of variables were described by canonical correlation analysis (CCA). Results showed a significant decrease of some bacteria or a complete removal after SLC (especially for P30). SLC also caused a decrease of %HDS and an increase of disulfide bridges and low and medium mBBr populations, suggesting the removal of immature sperm (poor chromatin compaction). CCA showed an association pattern compatible with the degradation of sperm chromatin parameters with bacterial contamination, especially Enterobacteria, P. aeuriginosa, and K. variicola. In conclusion, bacterial contamination affects sperm chromatin beyond DNA fragmentation; SLC with low-density colloid not only removes bacteria from boar semen, but also chromatin structure is enhanced after selection.(c) 2023 Published by Elsevier Inc.

Nyckelord

Boar; DNA fragmentation; Chromatin; Canonical correlation analysis; Single layer centrifugation

Publicerad i

Theriogenology
2023, Volym: 201, sidor: 95-105
Utgivare: ELSEVIER SCIENCE INC

SLU författare

• Morrell, Jane

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

Associerade SLU-program

AMR: Bakterier


Globala målen

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UKÄ forskningsämne

Klinisk vetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.theriogenology.2023.02.017

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/121824