Sammanfattning

Bovine respiratory syncytial virus (BRSV) is a pathogenic pneumovirus and a major cause of acute respiratory infections in calves. Although different vaccines are available against BRSV, their efficiency remains limited, and no efficient and large-scale treatment exists. Here, we developed a new reverse genetics system for BRSV expressing the red fluorescent protein mCherry, based on a field strain isolated from a sick calf in Sweden. Although this recombinant fluorescent virus replicated slightly less efficiently compared to the wild type virus, both viruses were shown to be sensitive to the natural steroidal alkaloid cyclopamine, which was previously shown to inhibit human RSV replication. Our data thus point to the potential of this recombinant fluorescent BRSV as a powerful tool in preclinical drug discovery to enable high throughput compound screening.

Nyckelord

Bovine respiratory syncytial virus; mCherry reverse genetics; antiviral drugs; cyclopamine

Publicerad i

Veterinary Research
2023, Volym: 54, nummer: 1, artikelnummer: 36
Utgivare: BMC

SLU författare

• Näslund, Katarina

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    
  • Sveriges Veterinärmedicinska Anstalt (SVA)

• Hägglund, Sara

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Valarcher, Jean-Francois

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

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UKÄ forskningsämne

Patobiologi


Publikationens identifierare

DOI: https://doi.org/10.1186/s13567-023-01165-x

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/122198