Sammanfattning

Bifidobacteria are among the most common bacteria used for their probiotic properties and their impact on the maturation and function of the immune system has been well-described. Recently, scientific interest is shifting from live bacteria to defined bacteria-derived biologically active molecules. Their greatest advantage over probiotics is the defined structure and the effect independent of the viability status of the bacteria. Here, we aim to characterize Bifidobacterium adolescentis CCDM 368 surface antigens that include polysaccharides (PSs), lip-oteichoic acids (LTAs), and peptidoglycan (PG). Among them, Bad368.1 PS was observed to modulate OVA-induced cytokine production in cells isolated from OVA-sensitized mice by increasing the production of Th1-related IFN-gamma and inhibition of Th2-related IL-5 and IL-13 cytokines (in vitro). Moreover, Bad368.1 PS (BAP1) is efficiently engulfed and transferred between epithelial and dendritic cells. Therefore, we propose that the Bad368.1 PS (BAP1) can be used for the modulation of allergic diseases in humans. Structural studies revealed that Bad368.1 PS has an average molecular mass of approximately 9,99 x 106 Da and it consists of glucose, galactose, and rhamnose residues that are creating the following repeating unit: [-> 2)-beta-D-Glcp-(1 -> 3)-beta-L-Rhap-(1 -> 4)-beta-D-Glcp-(1 -> 3)-alpha-L-Rhap-(1 -> 4)-beta-D-Glcp-(1 -> 3)-alpha-D-Galp-(1 -> ]n

Nyckelord

Allergy; Bifidobacterium; Polysaccharides; Surface antigens; Macromolecules

Publicerad i

Carbohydrate Polymers
2023, Volym: 315, artikelnummer: 120980
Utgivare: ELSEVIER SCI LTD

SLU författare

• Sandström, Corine

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Immunologi inom det medicinska området


Publikationens identifierare

DOI: https://doi.org/10.1016/j.carbpol.2023.120980

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/122785