Sammanfattning

BackgroundBiological variation (BV) of urinary (U) biochemical analytes has not been described in absolute terms, let alone as a ratio of the U-creatinine or fractional excretion in healthy dogs. These analytes are potential diagnostic tools for different types of kidney damage and electrolyte disorders in dogs. ObjectivesWe aimed to investigate the BV of specific gravity, osmolality, creatinine, urea, protein, glucose, chloride, sodium, potassium, calcium, and phosphate in urine from healthy pet dogs. MethodsBlood and urine samples from 13 dogs were collected once weekly for 8 weeks. Samples were analyzed in duplicate and in randomized order. For each sample, U-analyte and serum concentrations were measured, and U-analyte/U-creatinine and fractional excretion (FE) were calculated. Components of variance, estimated by restricted maximum likelihood, were used to determine within-subject variation (CVI), between-subject variation (CVG), and analytical variation (CVA). Index of individuality (II) and reference change values were calculated. ResultsCV(I) for all urine analytes varied between 12.6% and 35.9%, except for U-sodium, U-sodium/U-Cr, and FE-sodium, which had higher CV(I)s (59.5%-60.7%). For U-protein, U-sodium, U-potassium, U-sodium/U-creatinine, FE-urea, FE-glucose, FE-sodium, FE-potassium, and FE-phosphate II were low, indicating that population-based RIs were appropriate. The remaining analytes had an intermediate II, suggesting that population-based RIs should be used with caution. ConclusionThis study presents information on the biological variation of urinary and serum biochemical analytes from healthy dogs. These data are important for an appropriate interpretation of laboratory results.

Nyckelord

biomarker; canine; osmolality; renal; urine specific gravity

Publicerad i

Veterinary Clinical Pathology
2023, Volym: 52, nummer: 3, sidor: 461-474
Utgivare: WILEY

SLU författare

• Selin, Anna

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    
  • AniCura Djursjukhuset Albano

• Lilliehöök, Inger

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Forkman, Johannes

  • Institutionen för växtproduktionsekologi, Sveriges lantbruksuniversitet
    

• Pelander, Lena

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Strage, Emma

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Medicinsk biovetenskap


Publikationens identifierare

DOI: https://doi.org/10.1111/vcp.13225

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/122788