Belle, Simon
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
Översiktsartikel2021Vetenskapligt granskadÖppen tillgång
Lake Sedimentary DNA Research on Past Terrestrial and Aquatic Biodiversity: Overview and Recommendations
Capo, Eric; Giguet-Covex, Charline; Rouillard, Alexandra; Nota, Kevin; Heintzman, Peter D.; Vuillemin, Aurele; Ariztegui, Daniel; Arnaud, Fabien; Belle, Simon; Bertilsson, Stefan; Bigler, Christian; Bindler, Richard; Brown, Antony G.; Clarke, Charlotte L.; Crump, Sarah E.; Debroas, Didier; Englund, Goran; Ficetola, Gentile Francesco; Garner, Rebecca E.; Gauthier, Joanna;
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Sammanfattning
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises.
Nyckelord
sedimentary ancient DNA; sedimentary DNA; lake sediments; paleolimnology; paleoecology; paleogenetics; paleogenomics; metabarcoding; metagenomics; biodiversity
Publicerad i
Quaternary
2021, Volym: 4, nummer: 1, artikelnummer: 6
Utgivare: MDPI
Bertilsson, Stefan
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
SLU författare
UKÄ forskningsämne
Ekologi
Publikationens identifierare
DOI: https://doi.org/10.3390/quat4010006
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/123284