Sammanfattning

Human adenovirus 36 (HAdV-D36) is presently the sole virus identified to be associated with an elevated risk of obesity in both humans and animals. However, its impact on embryonated chicken eggs (ECEs) remains unexplored. This study endeavoured to examine the influence of HAdV-D36 on embryonic development by utilizing embryonated chicken eggs as a dynamic model. To simulate various infection routes, the allantoic cavity and the yolk sac of ECEs were inoculated with HAdV-D36. Subsequently, embryos from both the experimental (inoculated with virus) and control (inoculated with PBS) groups were weighed and subjected to daily histological examination. The daily embryo weights were assessed and compared between groups using the Shapiro-Wilk test. Histopathological changes in tissues were examined and compared between the tested and control groups to ascertain physiological alterations induced by the virus. Our study confirmed a significant increase in the body weight of ECEs. However, this phenomenon was not attributable to adipose tissue development; rather, it was characterized by an augmented number of cells in all observed tissues compared to control subjects. We posit that HAdV-D36 may impact developing organisms through mechanisms other than enhanced adipose tissue development. Specifically, our findings indicate an increased number of cells in all tissues, a phenomenon that occurs through an as-yet-unexplored pathway.

Nyckelord

embryonated chicken eggs; human adenovirus 36; organogenesis

Publicerad i

International Journal of Molecular Sciences
2024, Volym: 25, nummer: 4, artikelnummer: 2341
Utgivare: MDPI

SLU författare

• Stygar Österlund, Dominika

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    
  • Medical University Silesia

UKÄ forskningsämne

Cell- och molekylärbiologi
Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.3390/ijms25042341

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/128971