Sammanfattning

Truly comprehensive proteome analysis is highly desirable in systems biology and biomarker discovery efforts. But complete proteome characterization has been hindered by the dynamic range and detection sensitivity of experimental designs, which are not adequate to the very wide range of protein abundances. Experimental designs for comprehensive analytical efforts involve separation followed by mass spectrometry-based identification of digested proteins. Because results are generally reported as a collection of identifications with no information on the fraction of the proteome that was missed, they are difficult to evaluate and potentially misleading. Here we address this problem by taking a holistic view of the experimental design and using computer simulations to estimate the success rate for any given experiment. Our approach demonstrates that simple changes in typical experimental designs can enhance the success rate of proteome analysis by five- to tenfold

Publicerad i

Nature Biotechnology
2007, Volym: 25, nummer: 6, sidor: 651-655
Utgivare: NATURE PUBLISHING GROUP

SLU författare

• Eriksson, Jan

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

Publikationens identifierare

DOI: https://doi.org/10.1038/nbt1315

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/15683