Widenfalk, Anneli
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
Forskningsartikel2008Vetenskapligt granskad
Widenfalk, Anneli; Bertilsson, Stefan; Sundh, Ingvar; Goedkoop, Willem
A freshwater sediment was exposed to the pesticides captan, glyphosate, isoproturon, and pirimicarb at environmentally relevant and high concentrations. Effects on sediment microorganisms were studied by measuring bacterial activity, fungal and total microbial biomass as community-level endpoints. At the sub-community level, microbial community structure was analysed (PLFA composition and bacterial 16S rRNA genotyping, T-RFLP). Community-level endpoints were not affected by pesticide exposure. At lower levels of microbial community organization, however, molecular methods revealed treatment-induced changes in community composition. Captan and glyphosate exposure caused significant shifts in bacterial community composition (as T-RFLP) at environmentally relevant concentrations. Furthermore, differences in microbial community composition among pesticide treatments were found, indicating that test compounds and exposure concentrations induced multidirectional shifts. Our study showed that community-level end points failed to detect these changes, underpinning the need for application of molecular techniques in aquatic ecotoxicology. (c) 2007 Elsevier Ltd. All rights reserved.
pesticides; sediment; microbial communities; PLEA; genetic fingerprinting
Environmental Pollution
2008, Volym: 152, nummer: 3, sidor: 576-584
Utgivare: ELSEVIER SCI LTD
Fisk- och akvakulturforskning
Miljö- och naturvårdsvetenskap
DOI: https://doi.org/10.1016/j.envpol.2007.07.003
https://res.slu.se/id/publ/17859