Helmersson, Andreas
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Forskningsartikel2008Vetenskapligt granskad
Helmersson, Andreas; Jansson, Gunnar; Bozhkov, Peter V.; Von Arnold, Sara
Clonal propagation by somatic embryogenesis is a promising approach to preserve and multiply elite genotypes in tree breeding and reforestation programmes of conifers. The principal requirement for the large-scale application of this approach is near uniformity of the plants regenerated from somatic embryos. Picea abies families varied significantly in genetic stability, as shown by allele frequencies at four variable nuclear microsatellite loci in 314 plants regenerated from somatic embryos from six families. Furthermore, in some clones only one or two plants carried the mutated allele, while in other clones all the plants carried the same mutation. Relative growth increment was not affected in somatic embryo plants carrying mutated microsatellite alleles. For comparison, seedlings from half-sib families were analysed for microsatellite variation. Mutated alleles were detected in three of the 208 seedlings analysed. The allelic distribution of microsatellites in clones derived from somatic embryos was not significantly different from that of the parents. This suggests that the procedure for somatic embryogenesis does not give any large changes in allele frequency.
cloning; microsatellite markers; somatic embryogenesis
Scandinavian Journal of Forest Research
2008, Volym: 23, nummer: 1, sidor: 2-11
SDG15 Skydda, återställa och främja ett hållbart nyttjande av landbaserade ekosystem, hållbart bruka skogar, bekämpa ökenspridning, hejda och vrida tillbaka markförstöringen samt hejda förlusten av biologisk mångfald
Skogsvetenskap
DOI: https://doi.org/10.1080/02827580701820043
https://res.slu.se/id/publ/18417