Sammanfattning

Mast cells are implicated in rheumatoid arthritis, but the mechanism by which they contribute to disease progression is not clarified. Here we investigated whether mouse mast cell protease-4 (mMCP-4), a chymase present in the mast cell secretory granule, contributes to experimental arthritis. Two models of arthritis were investigated in mMCP-4(+/+) and mMCP-4(-/-) DBA/1 mice: collagen-induced arthritis ( CIA) was induced by immunization with collagen II (CII) in Freund's complete adjuvant, and a passive model of arthritis was induced by administration of anti-CII antibodies. The clinical scores were significantly reduced in the mMCP-4(-/-) animals as compared to mMCP-4(+/+) controls in both arthritis models. In CIA, the number of affected paws was lower in the CII-immunized mMCP-4(-/-) mice, with less cartilage destruction, pannus formation, and mononuclear cell and mast cell influx in the mMCP-4(-/-) joints. Interestingly, the lower clinical scores in the CII-immunized mMCP-4(-/-) mice coincided with lower serum levels of immunoglobulin G anti-CII antibodies. Our findings identify a pathogenic role of mMCP-4 in autoimmune arthritis.-Magnusson, S. E., Pejler, G., Kleinau, S., Abrink, M. Mast cell chymase contributes to the antibody response and the severity of autoimmune arthritis. FASEB J. 23, 875-882 (2009)

Nyckelord

protease and knockout mice; innate immune cell

Publicerad i

FASEB Journal
2009, Volym: 23, nummer: 3, sidor: 875-882

SLU författare

• Pejler, Gunnar

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Husdjursvetenskap
Veterinärmedicin


Publikationens identifierare

DOI: https://doi.org/10.1096/fj.08-120394

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/22655