Sammanfattning

The yeast Snf1 protein kinase and its animal homologue, the AMP-activated protein kinase, play important roles in metabolic regulation, by serving as energy gauges that turn off energy-consuming processes and mobilize energy reserves during low-energy conditions. The closest homologue of these kinases in plants is Snf1-related protein kinase 1 (SnRK1). We have cloned two SnRK1-encoding genes, PpSNF1a and PpSNF1b, in the moss Physcomitrella patens, where gene function can be studied directly by gene targeting in the haploid gametophyte. A snf1a snf1b double knockout mutant is viable, but lacks all Snf1-like protein kinase activity. The mutant has a complex phenotype that includes developmental abnormalities, premature senescence and altered sensitivities to plant hormones. Remarkably, the double knockout mutant also requires continuous light, and is unable to grow in a normal day-night light cycle. This suggests that SnRK1 is needed for metabolic changes that help the plant cope with the dark hours of the night

Publicerad i

EMBO Journal
2004, Volym: 23, nummer: 8, sidor: 1900-1910
Utgivare: NATURE PUBLISHING GROUP

SLU författare

• Thelander, Mattias

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Ronne, Hans

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Olsson, Tina

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

Publikationens identifierare

DOI: https://doi.org/10.1038/sj.emboj.7600182

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/2308