Unell, Maria
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Forskningsartikel2009Vetenskapligt granskadÖppen tillgång
Unell, Maria; Abraham, Paul E.; Shah, Manesh; Zhang, Bing; Rueckert, Christian; VerBerkmoes, Nathan C.; Jansson, Janet K.
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Arthrobacter chlorophenolicus; 4-chlorophenol; 4-nitrophenol; phenol; shotgun proteomics; 2D-LC-ES-MS/MS; hydroxyquinol dioxygenase
Journal of Proteome Research
2009, Volym: 8, nummer: 4, sidor: 1953-1964
Miljö- och naturvårdsvetenskap
DOI: https://doi.org/10.1021/pr800897c
https://res.slu.se/id/publ/26199