Sammanfattning

Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15-24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal. rhythm during a 24-h cycle. (C) 2008 Elsevier GmbH. All rights reserved.

Nyckelord

Barley; Endosperm; Oscillation; SBE; Sorghum

Publicerad i

Journal of Plant Physiology
2009, Volym: 166, nummer: 4, sidor: 428-434

SLU författare

• Mutisya, Joel

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    
  • Kenya Agricultural Research Institute

• Sun, Chuanxin

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Rosenquist, Sara

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Jansson, Christer

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Livsmedelsvetenskap
Jordbruksvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.jplph.2008.06.017

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/28884