Sammanfattning

A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta(2)-glycoprotein I (beta(2)-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta(2)-GPI was also detected on all tested CVCs

Nyckelord

Staphylococcus aureus; phage display; biomaterial; receptin; beta 2-glycoprotein I

Publicerad i

Journal of Medical Microbiology
2004, Volym: 53, nummer: 10, sidor: 945-951
Utgivare: SOC GENERAL MICROBIOLOGY

SLU författare

• Bjerketorp, Joakim

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Frykberg, Lars

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Jacobsson, Karin

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

Publikationens identifierare

DOI: https://doi.org/10.1099/jmm.0.45638-0

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/3036