Gohil, Suresh
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Forskningsartikel2004Vetenskapligt granskad
Synthesis, physicochemical and biochemical studies of 1',2'-oxetane constrained adenosine and guanosine modified oligonucleotides, and their comparison with those of the corresponding cytidine and thymidine analogues
Pradeepkumar PI, Cheruku P, Plashkevych O, Acharya P, Gohil S, Chattopadhyaya J
Sammanfattning
We have earlier reported the synthesis and antisense properties of the conformationally constrained oxetane-C and -T containing oligonucleotides, which have shown effective down-regulation of the proto-oncogene c-myb mRNA in the K562 human leukemia cells. Here we report on the straightforward syntheses of the oxetane-A and oxetane-G nucleosides as well as their incorporations into antisense oligonucleotides (AONs), and compare their structural and antisense properties with those of the T and C modified AONs (including the thermostability and RNase H recruitment capability of the AON/RNA hybrid duplex by Michaelis-Menten kinetic analyses, their resistance in the human serum, as well as in the presence of exo and endonucleases)
Publicerad i
Journal of the American Chemical Society
2004, Volym: 126, nummer: 37, sidor: 11484-11499
Utgivare: AMER CHEMICAL SOC
SLU författare
Publikationens identifierare
DOI: https://doi.org/10.1021/ja0484171
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/3137