Sammanfattning

We have developed a protocol for rapid generation of barley plants with introduced genes by employing techniques of tissue culture, plant regeneration and crossing. Compared to conventional protocols, the time between pollination of To plants and identification of phenotypic expression in the T(1) generation is reduced from around 17-9 weeks. All of the selected candidates after the tissue culture screening exhibited phenotypic expression. Furthermore, by utilizing ordinary pollen crossing, we demonstrated a route for introduction of genes to a recalcitrant barley elite cultivar. This Tissue Culture after Crossing (TCC) procedure significantly enhances the prospects for producing transgenic barley lines from desirable germplasm. We suggest that the TCC method will contribute to improve barley as a promising bioenergy crop. (C) 2011 Elsevier Ltd. All rights reserved.

Nyckelord

Barley; Bioenergy; Tissue culture; Transgenics

Publicerad i

Applied Energy
2012, Volym: 91, nummer: 1, sidor: 405-411

SLU författare

• Nalawade, Satish

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    
  • Mahyco Research Center

• Sun, Chuanxin

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Jordbruksvetenskap
Förnyelsebar bioenergi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.apenergy.2011.09.042

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/37552