Sandgren, Mats
- Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
Forskningsartikel2011Vetenskapligt granskadÖppen tillgång
Sandgren, Mats; Wu, Miao; Ishida, Takuya; Ståhlberg, Jerry
Many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (GH61), some of which are known to act synergistically with cellulases. In this study we show that PcGH61D, the gene product of an open reading frame in the genome of Phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to generation of aldonic acids. The activity of this enzyme and its beneficial effect on the efficiency of classical cellulases are stimulated by the presence of electron donors. Experiments with reduced cellulose confirmed the oxidative nature of the reaction catalyzed by PcGH61D and indicated that the enzyme may be capable of penetrating into the substrate. Considering the abundance of GH61-encoding genes in fungi and genes encoding their functional bacterial homologues currently classified as carbohydrate binding modules family 33 (CBM33), this enzyme activity is likely to turn out as a major determinant of microbial biomass-degrading efficiency.
PLoS ONE
2011, Volym: 6, nummer: 11
Utgivare: PUBLIC LIBRARY SCIENCE
Biokemi och molekylärbiologi
DOI: https://doi.org/10.1371/journal.pone.0027807
https://res.slu.se/id/publ/40783