Sammanfattning

We have previously generated an affibody molecule for the disease-associated amyloid beta (A beta) peptide, which has been shown to inhibit the formation of various A beta aggregates and revert the neurotoxicity of A beta in a fruit fly model of Alzheimer's disease. In this study, we have investigated a new bacterial display system for combinatorial protein engineering of the A beta-binder as a head-to-tail dimeric construct for future optimization efforts, e.g. affinity maturation. Using the bacterial display platform, we have: (i) demonstrated functional expression of the dimeric binder on the cell surface, (ii) determined the affinity and investigated the pH sensitivity of the interaction, (iii) demonstrated the importance of an intramolecular disulfide bond through selections from a cell-displayed combinatorial library, as well as (iv) investigated the effects from rational truncation of the N-terminal part of the affibody molecule on surface expression level and A beta binding. Overall, the detailed engineering and characterization of this promising A beta-specific affibody molecule have yielded valuable insights concerning its unusual binding mechanism. The results also demonstrated that our bacterial display system is a suitable technology for future protein engineering and characterization efforts of homo- or heterodimeric affinity proteins.

Nyckelord

Affibody molecules; Alzheimer's disease; Amyloid beta; Bacterial display; Combinatorial protein engineering

Publicerad i

Biotechnology Journal
2013, Volym: 8, nummer: 1, sidor: 139-145
Utgivare: WILEY-BLACKWELL

SLU författare

• Härd, Torleif

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Läkemedelsbioteknik
Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1002/biot.201200228

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/40821