Sammanfattning

The physiological role of the Norway spruce [Picea abies (L.) Karst.] spi 2 gene, encoding a defense-related cationic peroxidase was examined in transgenic tobacco (Nicotiana tabacum L.). Expression of spi 2, under control of the 35S promoter., in tobacco plants resulted in higher total peroxidase activities. The phenotype of the spi 2-transformed lines was normal. The spi 2-transformed lines displayed lignin levels similar to levels in the control line, but with some alteration in lignin histochemistry and structure. These changes were associated with reduced flexibility of the tobacco stems. The defense against pathogenic microorganisms was altered in the transgenic tobacco plants compared with control plants. High peroxidase activities increased the susceptibility to the pathogenic oomycete Phytophthora parasitica var. nicotianae, but increased the ability of the tobacco plants to suppress growth of the pathogenic bacterium Enwinia carotovora.

Nyckelord

coniferaldehyde; defense; Erwinia; lignin; Nicotiana; peroxidase; Phytophtora

Publicerad i

Planta
2002, Volym: 214, nummer: 5, sidor: 708-716

SLU författare

• Elfstrand, Malin

  • Institutionen för skogsgenetik, Sveriges lantbruksuniversitet
    

• Sitbon, Folke

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Von Arnold, Sara

  • Institutionen för skogsgenetik, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Genetik och förädling
Biokemi och molekylärbiologi
Jordbruksvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1007/s00425-001-0681-5

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/42955