Sammanfattning

Proteomic effect screening in zebrafish liver was performed to generate hypotheses following exposure (21 days) to a structurally diverse mixture of brominated flame retardants (BFRs). Fish were exposed to two doses (10 and 100 nmol/g feed). Two-dimensional gel-electrophoresis, image analysis and MALDI-TOF mass-spectrometry revealed 13 and 19 significant responses in males and females, respectively. Effects on proteins related to cellular maintenance and stress were observed in both genders. Regulated proteins were gender-specific, but functionally indicated common protective responses (peroxiredoxin 6 and Zgc:92891 in males and transketolase in females) suggesting oxidative stress. Betaine homocysteine methyltransferase (BHMT) was induced in both genders. In addition a female-specific downregulation of iron homeostatic proteins (iron-regulatory protein 1 and transferfin) were observed. Our proteomic approach revealed novel responses that suggest important gender-specific sensitiviy to BFRs that should be considered when interpreting adverse effects of BFRs. (c) 2007 Elsevier Inc. All rights reserved.

Nyckelord

fish; proteomics; BFR; MALDI-TOF

Publicerad i

Ecotoxicology and Environmental Safety
2008, Volym: 71, nummer: 2, sidor: 319-327
Utgivare: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU författare

• Norman Haldén, Anna

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Norrgren, Leif

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

Globala målen

SDG14 Bevara och nyttja haven och de marina resurserna på ett hållbart sätt för en hållbar utveckling


UKÄ forskningsämne

Patobiologi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.ecoenv.2007.12.010

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/43006