A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Svačinová, Jana; Novak, Ondrej; Plačková, Lenka; Lenobel, René; Holík, Josef; Strnad, Miroslav; Doležal, Karel

doi:10.1186/1746-4811-8-17

Forskningsartikel2012Vetenskapligt granskadÖppen tillgång

A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Svačinová, Jana; Novak, Ondrej; Plačková, Lenka; Lenobel, René; Holík, Josef; Strnad, Miroslav; Doležal, Karel

Sammanfattning

Conclusions: The combination of microextraction with one-step high-throughput purification provides fast, effective and cheap sample preparation prior to qualitative and quantitative measurements. Our procedure can be used after modification also for other phytohormones, depending on selectivity, affinity and capacity of the selected sorbents.

Nyckelord

Cytokinins; Arabidopsis thaliana; Pipette tip solid-phase extraction (PT-SPE); StageTip; Ultra-high; performance liquid chromatography (UHPLC); Tandem mass spectrometry (MS/MS)

Publicerad i

Plant Methods
2012, Volym: 8
Utgivare: BIOMED CENTRAL LTD

SLU författare

Novak, Ondrej
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet

UKÄ forskningsämne

Botanik

Publikationens identifierare

DOI: https://doi.org/10.1186/1746-4811-8-17

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/44832