Sammanfattning

A species specific forward primer was designed in order to identify the phytopathogenic fungus Alternaria solani, which causes early blight on potato. The reverse primer came from another survey of the American population of A. solani. The combination of these two primers can be used both as molecular identification of A. solani as well as sending the PCR product for sequencing in search for the F129L substitution associated with loss of sensitivity toward fungicides based on strobilurins. The diagnostic method has been successfully used when identifying the causal agent of lesions on potato leaflets during large field assays. (C) 2012 Elsevier Ltd. All rights reserved.

Nyckelord

Molecular diagnostics; PCR; Species-specific primers; Fungicide resistance; Alternaria solani

Publicerad i

Crop Protection
2012, Volym: 38, sidor: 72-73
Utgivare: ELSEVIER SCI LTD

SLU författare

• Edin, Eva

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

Associerade SLU-program

AMR: Svampar


UKÄ forskningsämne

Jordbruksvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.cropro.2012.03.021

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/45305