Hellman, Maria
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Forskningsartikel2011Vetenskapligt granskad
Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates
Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.; Hallin, Sara
Sammanfattning
Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.
Nyckelord
Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog
Publicerad i
Journal of Microbiological Methods
2011, Volym: 86, nummer: 3, sidor: 376-378
Utgivare: ELSEVIER SCIENCE BV
Hallin, Sara
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
SLU författare
UKÄ forskningsämne
Jordbruksvetenskap
Fisk- och akvakulturforskning
Miljö- och naturvårdsvetenskap
Publikationens identifierare
DOI: https://doi.org/10.1016/j.mimet.2011.05.020
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/46481