Sammanfattning

In this study the design and development of two real-time PCR assays for the rapid, sensitive and specific detection of infectious laryngotracheitis virus (ILTV) DNA is described. A Primer-Probe Energy Transfer (PriProET) assay and 5' conjugated Minor Groove Binder (MGB) method are compared and contrasted. Both have been designed to target the thymidine kinase gene of the ILTV genome. Both PriProET and MGB assays are capable of detecting 20 copies of a DNA standard per reaction and are linear from 2 x 10(8) to 2 x 10(2) copies/mu l. Neither PriProET, nor MGB reacted with heterologous herpesviruses, indicating a high specificity of the two methods as novel tools for virus detection and identification. This study demonstrates the suitability of PriProET and 5' conjugated MGB probes as real-time PCR chemistries for the diagnosis of respiratory diseases caused by ILTV. (C) 2011 Elsevier B.V. All rights reserved.

Nyckelord

Infectious laryngotracheitis virus; Real-time PCR; Primer-Probe Energy Transfer; Minor Groove Binder probe

Publicerad i

Journal of Virological Methods
2011, Volym: 175, nummer: 2, sidor: 149-155
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Belak, Sandor

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Husdjursvetenskap
Veterinärmedicin


Publikationens identifierare

DOI: https://doi.org/10.1016/j.jviromet.2011.04.020

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/46515