Skip to main content
SLU:s publikationsdatabas (SLUpub)

Forskningsartikel2004Vetenskapligt granskad

Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcl component of Streptomyces clavuligerus 7 alpha-cephem-methoxylase

Lester DR, Oster LM, Svenda M, Andersson I

Sammanfattning

Cephamycins are broad-spectrum beta-lactam antibiotics that show resistance to certain forms of beta-lactamases. They differ from cephalosporins by the presence of a methoxyl group at the C-7alpha position. The gene products of cmcI and cmcJ are believed to control 7alpha-methoxylation of cephalosporins through successive steps of hydroxylation and methylation. Here, the expression, purification, crystallization and initial data-collection statistics of the 236-amino-acid protein product of cmcI from Streptomyces clavuligerus is reported. The crystals belong to space group P2(1), with unit-cell parameters a = 93.6, b = 182.6, c = 103.2 Angstrom, beta = 91.05degrees. Diffraction data were collected to 2.5 Angstrom

Publicerad i

Acta Crystallographica Section D: Biological Crystallography
2004, Volym: 60, sidor: 1618-1621
Utgivare: BLACKWELL MUNKSGAARD

      SLU författare

    • Lester, Diane

      • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet

      • Andersson, Inger

        • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet

        • Öster, Linda

          • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet

        Publikationens identifierare

        DOI: https://doi.org/10.1107/S090744490401666X

        Permanent länk till denna sida (URI)

        https://res.slu.se/id/publ/4781