Sammanfattning

Selected Reaction Monitoring (SRM) is a method of choice for accurate quantitation of low-abundance proteins in complex backgrounds. This strategy is, however, sensitive to interference from other components in the sample that have the same precursor and fragment masses as the monitored transitions. We present here an approach to detect interference by using the expected relative intensity of SRM transitions. We also designed an algorithm to automatically detect the linear range of calibration curves. These approaches were applied to the experimental data of Clinical Proteomic Tumor Analysis Consortium (CPTAC) Verification Work Group Study 7 and show that the corrected measurements provide more accurate quantitation than the uncorrected data. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.

Nyckelord

Mass spectrometry; Selected reaction monitoring; Interference detection; Interference correction; Linear range detection

Publicerad i

Methods
2013, Volym: 61, nummer: 3, sidor: 299-303
Utgivare: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU författare

• Eriksson, Jan

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Bioinformatik och systembiologi
Analytisk kemi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.ymeth.2013.05.008

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/50297