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Forskningsartikel1998Vetenskapligt granskad

Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation

Lee, Michael; Lenman, Marit; Banas, Antoni; Bafor, Maureen; Surinder, Singh; Schweizer, Michael; Nilsson, Ralf; Liljenberg, Conny; Dahlqvist, Anders; Gummeson, Per-Olov; Sjödahl, Staffan; Green, Allan; Stymne, Sten

Sammanfattning

Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta 12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta 12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.

Publicerad i

Science
1998, Volym: 280, nummer: 5365, sidor: 915-918
Utgivare: AMER ASSOC ADVANCEMENT SCIENCE

      SLU författare

    • Lenman, Marit

      • Institutionen för växtförädling, Sveriges lantbruksuniversitet

      • Banas, Antoni

        • Institutionen för växtförädling, Sveriges lantbruksuniversitet

        • Dahlqvist, Anders

          • Institutionen för växtförädling, Sveriges lantbruksuniversitet

          • Stymne, Sten

            • Institutionen för växtförädling, Sveriges lantbruksuniversitet

          UKÄ forskningsämne

          Växtbioteknologi
          Cellbiologi

          Publikationens identifierare

          DOI: https://doi.org/10.1126/science.280.5365.915

          Permanent länk till denna sida (URI)

          https://res.slu.se/id/publ/52295