Sammanfattning

A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.

Nyckelord

ARABIDOPSIS-THALIANA; BRASSICACEAE; BRASSICA-NAPUS; GLUCOSINOLATE; MYROSINASE; SINAPIS-ALBA

Publicerad i

Plant Molecular Biology
1992, Volym: 18, nummer: 2, sidor: 387-398
Utgivare: KLUWER ACADEMIC PUBL

SLU författare

• Lenman, Marit

  • Institutionen för cellforskning, Sveriges lantbruksuniversitet
    

• Falk, Anders

  • Institutionen för cellforskning, Sveriges lantbruksuniversitet
    

• Rask, Lars

  • Institutionen för cellforskning, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Växtbioteknologi


Publikationens identifierare

DOI: https://doi.org/10.1007/BF00034965

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/52303