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Research article2002Peer reviewed

Crystallization and X-ray analysis of native and selenome­thionyl β-mannanase Man5A from blue mussel, Mytilus edulis, expressed in Pichia pastoris

Xu, Bingze; Munoz, Ines G.; Janson, Jan-Christer; Ståhlberg, Jerry

Abstract

The glycohydrolase family 5 beta-mannanase Man5A from Mytilus edulis has been expressed in Pichia pastoris and purified in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop technique at 293 K using polyethylene glycol 5000 monomethylether as precipitant and dioxane as additive. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.8, b = 64.8, c = 90.7 Angstrom. Diffraction to 1.4 Angstrom resolution has been obtained at 100 K. Expression was also performed in the presence of selenomethionine. The incorporation of SeMet was estimated at 40% by amino-acid analysis and its presence in crystals was confirmed from the X-ray absorption scanning spectrum.

Published in

Acta Crystallographica Section D: Biological Crystallography
2002, Volume: 58, pages: 542-545
Publisher: BLACKWELL MUNKSGAARD

      SLU Authors

    • Munoz, Ines G.

      • Department of Molecular Biology, Swedish University of Agricultural Sciences

      • Ståhlberg, Jerry

        • Department of Molecular Biology, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Structural Biology

      Publication identifier

      DOI: https://doi.org/10.1107/S0907444902000355

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/53155