Sammanfattning

Dextranase from the fungus Penicillium minioluteum hydrolyses alpha-1,6-glycosidic bonds in dextran polymers. The enzyme has been expressed in Pichia pastoris in the presence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by amino-acid analysis to be 50%. The protein has been crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Angstrom and one molecule per asymmetric unit. The crystals diffract to 2.0 Angstrom and the presence of SeMet in the crystals has been confirmed by an X-ray absorption spectrum.

Publicerad i

Acta Crystallographica Section D: Biological Crystallography
2002, Volym: 58, sidor: 346-348
Utgivare: BLACKWELL MUNKSGAARD

SLU författare

• Ståhlberg, Jerry

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Biokemi och molekylärbiologi
Strukturbiologi


Publikationens identifierare

DOI: https://doi.org/10.1107/S0907444901020406

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/53162