Sammanfattning

In a flash diffraction experiment, a short and extremely intense x-ray pulse illuminates the sample to obtain a diffraction pattern before the onset of significant radiation damage. The over-sampled diffraction pattern permits phase retrieval by iterative phasing methods. Flash diffractive imaging was first demonstrated on an inorganic test object (Chapman et al 2006 Nat. Phys. 2 839-43). We report here experiments on biological systems where individual cells were imaged, using single, 10-15 fs soft x-ray pulses at 13.5 nm wavelength from the FLASH free-electron laser in Hamburg. Simulations show that the pulse heated the sample to about 160 000 K but not before an interpretable diffraction pattern could be obtained. The reconstructed projection images return the structures of the intact cells. The simulations suggest that the average displacement of ions and atoms in the hottest surface layers remained below 3 angstrom during the pulse.

Publicerad i

Journal of Physics B: Atomic, Molecular and Optical Physics
2010, Volym: 43, nummer: 19, artikelnummer: 194015
Utgivare: IOP PUBLISHING LTD

SLU författare

• Andersson, Inger

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Atom- och molekylfysik och optik


Publikationens identifierare

DOI: https://doi.org/10.1088/0953-4075/43/19/194015

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/61210