Sammanfattning

Mitochondrial thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK) catalyze the initial phosphorylation of pyrimidine and purine deoxyribonucleosides, and are essential for maintaining mitochondrial dNTP pools for mitochondrial DNA replication. Here the expression of mitochondrial TK2 and dGK in relation to cell growth phases in cultured cells was investigated. TK2 and dGK protein levels in isolated mitochondria and TK2 activity in total cell extracts from U2OS and TK1 deficient L929 cells were determined. We found that TK2 levels were negatively correlated with cell growth rates and there was an exponential increase in TK2 levels in cells entering stationary phase. The expression of dGK did not change and appeared to be constitutive.

Nyckelord

Thymidine kinase 2; deoxyguanosine kinase; expression profile

Publicerad i

Nucleosides, Nucleotides and Nucleic Acids
2014, Volym: 33, nummer: 4-6, sidor: 282-286

SLU författare

• Sun, Ren

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Eriksson, Staffan

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Wang, Liya

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Cellbiologi
Biokemi och molekylärbiologi
Cell- och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1080/15257770.2013.854383

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/62167