Skip to main content
SLU:s publikationsdatabas (SLUpub)

Bokkapitel2015Vetenskapligt granskad

Metagenomic approaches to disclose disease-associated pathogens: detection of viral pathogens in honeybees

Granberg, Fredrik; Karlsson, Oskar; Belák, Sándor

Sammanfattning

Metagenomic approaches have become invaluable for culture-independent and sequence-independent detection and characterization of disease-associated pathogens. Here, the sequential steps from sampling to verification of results are described for a metagenomic-based approach to detect potential pathogens in honeybees. The pre-sequencing steps are given in detail, but due to the rapid development of sequencing technologies, all platform-specific procedures, as well as subsequent bioinformatics analysis, are more generally described. It should also be noted that this approach could, with minor modifications, be adapted for other organisms and sample matrices.

Nyckelord

Virus, metagenomics

Publicerad i

Methods in Molecular Biology
2015, Volym: 1247, nummer: 1247, sidor: 491-511
Titel: Veterinary infection biology: molecular diagnostics and high-throughput strategies Methods in Molecular Biology
ISBN: 978-1-4939-2004-4
Utgivare: Springer New York