Sammanfattning

The identification and cloning of full-length homologs of circadian clock genes from Picea abies represent a first step to study the function and evolution of the circadian clock in gymnosperms. Phylogenetic analyses suggest that the sequences of key circadian clock genes are conserved between angiosperms and gymnosperms. though fewer homologous copies were found for most gene families in P. abies. We detected diurnal cycling of circadian clock genes in P. abies using quantitative real-time PCR; however, cycling appeared to be rapidly dampened under free-running conditions. Given the unexpected absence of transcriptional cycling during constant conditions, we employed a complementary method to assay circadian rhythmic outputs and measured delayed fluorescence in seedlings of Norway spruce. Neither of the two approaches to study circadian rhythms in Norway spruce could detect robust similar to 24 h cycling behavior under constant conditions. These data suggest gene conservation but fundamental differences in clock function between gymnosperms and other plant taxa.

Nyckelord

Circadian clock; Delayed fluorescence; Diurnal cycling; Gene expression; Picea abies; Phylogeny

Publicerad i

Plant and Cell Physiology
2014, Volym: 55, nummer: 3, sidor: 535-550
Utgivare: OXFORD UNIV PRESS

SLU författare

• Gyllenstrand, Niclas

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Clapham, David

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Utvecklingsbiologi
Genetik
Växtbioteknologi


Publikationens identifierare

DOI: https://doi.org/10.1093/pcp/pct199

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/66867