Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

Balint  A, Balka  G, Horvath  P, Kecskemeti  S, Dan  A, Farsang  A, Szeredi  L, Banyai  K, Bartha  D, Olasz  F, Belak  S, Zadori  Z

doi:10.1007/s00705-014-2265-2

Forskningsartikel2015Vetenskapligt granskad

Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

Balint A, Balka G, Horvath P, Kecskemeti S, Dan A, Farsang A, Szeredi L, Banyai K, Bartha D, Olasz F, Belak S, Zadori Z

Sammanfattning

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

Nyckelord

PRRSV; Sequencing; Complete genome; Epitope

Publicerad i

Archives of Virology
2015, Volym: 160, nummer: 2, sidor: 417-422

UKÄ forskningsämne

Medicinsk biovetenskap

Publikationens identifierare

DOI: https://doi.org/10.1007/s00705-014-2265-2

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/67642