Sammanfattning

Changes in serum metabolic profile after the intake of different food products (e.g., bread) can provide insight into their interaction with human metabolism. Postprandial metabolic responses were compared after the intake of refined wheat (RWB), whole-meal rye (WRB), and refined rye (RRB) breads. In addition, associations between the metabolic profile in fasting serum and the postprandial concentration of insulin in response to different breads were investigated. Nineteen postmenopausal women. with normal fasting glucose and normal glucose tolerance participated in a randomized, controlled, crossover meal study. The test breads, RWB (control), RRB, and WRB, providing 50 g of available carbohydrate, were each served as a single meal. The postprandial metabolic profile was measured using nuclear magnetic resonance and targeted LC-mass spectrometry and was compared between different breads using ANOVA and multivariate models. Eight amino acids had a significant treatment effect (P < 0.01) and a significant treatment x time effect (P < 0.05). RWB produced higher postprandial concentrations of leucine (geometric mean: 224; 95% Cl: 196, 257) and isoleucine (mean +/- SD: 111 +/- 31.5) compared with RRB (geometric mean: 165; 95% Cl: 147, 186; mean +/- SD: 84.2 +/- 22.9) and WRB (geometric mean: 190; 95% Cl: 174, 207; mean +/- SD: 95.8 +/- 17.3) at 60 min respectively (P < 0.001). In addition, 2 metabolic subgroups were identified using multivariate models based on the association between fasting metabolic profile and the postprandial concentration of insulin. Women with higher fasting concentrations of leucine and isoleucine and lower fasting concentrations of sphingomyelins and phosphatidylcholines had higher insulin responses despite similar glucose concentration after all kinds of bread (cross-validated ANOVA, P = 0.048). High blood concentration of branched-chain amino acids, i.e., leucine and isoleucine, has been associated with the increased risk of diabetes, which suggests that additional consideration should be given to bread proteins in understanding the beneficial health effects of different kinds of breads. The present study suggests that the fasting metabolic profile can be used to characterize the postprandial insulin demand in individuals with normal glucose metabolism that can be used for establishing strategies for the stratification of individuals in personalized nutrition.

Publicerad i

Journal of Nutrition
2014, Volym: 144, nummer: 6, sidor: 807-814
Utgivare: AMER SOC NUTRITION-ASN

SLU författare

• Moazzami, Ali

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Shrestha, Aahana

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Morrison, David

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

Globala målen

Säkerställa hälsosamma liv och främja välbefinnande för alla i alla åldrar
Avskaffa hunger, uppnå tryggad livsmedelsförsörjning och förbättrad nutrition samt främja ett hållbart jordbruk


UKÄ forskningsämne

Näringslära
Organisk kemi


Publikationens identifierare

DOI: https://doi.org/10.3945/jn.113.188912

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/68002