Sammanfattning

The present work describes the feasibility of a cross-linkable injectable hyaluronan hydrogel for cartilage repair. The hydrogel used is a two-component system based on aldehyde-modified hyaluronan and hydrazide-modified polyvinyl alcohol, which are rapidly cross-linked in situ upon mixing. The in vitro study showed that chondrocytes and mesenchymal cells cultured in the gel form cartilage-like tissue, rich in glycosaminoglycans, collagen type II and aggrecan. In a rabbit animal model the injection of the hydrogel improved the healing of a full-thickness cartilage defect created in the knee as compared to non-treated controls. This rabbit study showed that the regenerated cartilage defects stained more intensely for type II collagen upon treatment with the hydrogel. The hyaluronan-based hydrogel may be used as a delivery vehicle for both growth factors and/or cells for cartilage repair. The in vivo study also indicated that the hydrogel alone has a beneficial effect on cartilage regeneration. Copyright (C) 2011 John Wiley & Sons, Ltd.

Nyckelord

cartilage; injectable hyaluronan; cross-linked; bone morphogenetic protein-2; rabbit model

Publicerad i

Journal of Tissue Engineering and Regenerative Medicine
2011, Volym: 5, nummer: 8, sidor: E188-E196
Utgivare: WILEY-BLACKWELL

SLU författare

• Jensen Waern, Marianne

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Hedenqvist, Patricia

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Husdjursvetenskap
Veterinärmedicin
Medicinsk bioteknik


Publikationens identifierare

DOI: https://doi.org/10.1002/term.415

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/68312