Sammanfattning

The pine weevil Hylobius abietis is a severe pest of conifer seedlings in reforestation areas. Weevils lay eggs in the root bark or in the soil near roots of recently dead trees and cover the eggs with frass (feces combined with chewed bark), possibly to avoid conspecific egg predation. The aim of the present investigation focused on isolation, identification, and volatile production of fungi from pine-weevil feces and frass. Fungi were isolated from weevil frass and feces separately, followed by identification based on ITS sequencing. Fifty-nine isolates belonging to the genera Penicillium, Ophiostoma, Mucor, Leptographium, Eucasphaeria, Rhizosphaera, Debaryomyces, and Candida were identified. Volatile organic compounds (VOCs) produced by the fungal community and fungal isolates cultured on weevil-frass broth were identified by SPME-GCMS. Major VOCs emitted from the fungal community and pure isolates were species- and strain specific and included isopentylalcohol, styrene, 3-octanone, 6-protoilludene, methyl salicylate, 3-methylanisole, 2-methoxyphenol, and phenol. Some of these are known to influence the orientation of pine weevils when tested among highly attractive newly planted conifer seedlings. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Nyckelord

Candida sequanensis; Debaryomyces; Fungal community; Methyl salicylate; Ophiostoma; Penicillium

Publicerad i

Fungal Biology
2015, Volym: 119, nummer: 8, sidor: 738-746
Utgivare: ELSEVIER SCI LTD

SLU författare

• Terenius, Olle

  • Institutionen för ekologi, Sveriges lantbruksuniversitet
    

• Nordenhem, Henrik

  • Institutionen för ekologi, Sveriges lantbruksuniversitet
    

• Nordlander, Göran

  • Institutionen för ekologi, Sveriges lantbruksuniversitet
    

Globala målen

SDG15 Skydda, återställa och främja ett hållbart nyttjande av landbaserade ekosystem, hållbart bruka skogar, bekämpa ökenspridning, hejda och vrida tillbaka markförstöringen samt hejda förlusten av biologisk mångfald


UKÄ forskningsämne

Mikrobiologi
Ekologi
Organisk kemi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.funbio.2015.04.008

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/68393