Sammanfattning

Mast cells (MCs) have detrimental functions in the context of numerous pathologies, and regimens aimed at neutralizing MCs or individual MC products can thus be of therapeutic value. One way to target MCs in disease is to selectively induce MC apoptosis, but there is so far no agent available that selectively induces apoptosis in MCs. Mast cells are heavily loaded with secretory granules containing large amounts of fully active proteases bound to serglycin proteoglycan. Damage to the secretory granules will thus lead to the release of serglycin-protease complexes into the cytosol. A potential consequence of this would be that the unleashed granular proteases cause apoptosis by proteolytic activation of proapoptotic compounds located in the cytosol. Indeed, we have recently found that MCs are highly sensitive to apoptosis induced by permeabilization of the secretory granules. In this chapter, we describe the methods used to study MC apoptosis induced by this novel, secretory granule-mediated pathway.

Nyckelord

Mast cells; Apoptosis; Proteases; Granules; Caspases

Publicerad i

Methods in Molecular Biology
2015, Volym: 1220, nummer: 1220, sidor: 325-337
Titel: Mast Cells : methods and protocols
ISBN: 978-1-4939-1567-5
Utgivare: Springer New York

SLU författare

• Rabelo Melo, Fabio

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Wernersson, Sara

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Pejler, Gunnar

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Cell- och molekylärbiologi
Medicinsk biovetenskap


Publikationens identifierare

DOI: https://doi.org/10.1007/978-1-4939-1568-2_20

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/68713