Sammanfattning

Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl(4)Ac(1) and GlcA-Xyl(5)Ac(2)) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls. (C) 2015 Elsevier B.V. All rights reserved.

Nyckelord

Arabidopsis thaliana; Glucuronoxylan; Tandem mass spectrometry

Publicerad i

International Journal of Biological Macromolecules
2015, Volym: 79, sidor: 807-812
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Derba-Maceluch, Marta

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Mellerowicz, Ewa

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.ijbiomac.2015.05.056

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/75552