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Forskningsartikel1995Vetenskapligt granskad

Development of a new chemical method for distinguishing between Betulapendula and Betulapubescens in Sweden

Lundgren, L. N.; Pan, H.; Theander, O.; Eriksson, Harry; Johansson, Ulf; Svenningsson, M.

Sammanfattning

High performance liquid chromatography (HPLC) analytical studies of inner bark extractives from a large number of silver birch (Betula pendula Roth) and hairy birch (Betula pubescens Ehrh.) trees revealed the diarylheptanoid glucoside platyphylloside to be present at high levels in B. pendula (20-60 mg/g dry bark) and at low levels in B. pubescens (less than or equal to 0.5 mg/g dry bark). Based on these observations, a new simple and accurate precipitation method to distinguish between B. pendula and B. pubescens has been developed. In a solution of 2,4-dinitrophenylhydrazine, platyphylloside immediately forms an orange precipitate. A similar precipitate is also produced when a piece of B. pendula inner bark is kept for 1 h at room temperature in the same solution. However, no precipitate forms when a sample from B. pubescens is used. The diagnostic value of morphological characters was assessed by comparing the results of species determinations made on a large number of trees (>1600), both juvenile and mature, using the morphological and the new chemical method. For birches younger than 20 years there was 100% agreement between the two methods. However, in some mature birch stands with the two species mixed and ages over 40 years, up to 20-30% of the trees were incorrectly identified to species using morphological characters. Results from tests using the precipitation method on birch species, varieties, forms, and hybrids were reported.

Publicerad i

Canadian Journal of Forest Research
1995, Volym: 25, nummer: 7, sidor: 1097-1102
Utgivare: NATL RESEARCH COUNCIL CANADA

      SLU författare

    • Eriksson, Harry

      • Institutionen för skogsproduktion, Sveriges lantbruksuniversitet
      • Johansson, Ulf

        • Institutionen för skogsproduktion, Sveriges lantbruksuniversitet

      UKÄ forskningsämne

      Skogsvetenskap

      Publikationens identifierare

      DOI: https://doi.org/10.1139/x95-121

      Permanent länk till denna sida (URI)

      https://res.slu.se/id/publ/79205