Sammanfattning

A gene encoding a fibrinogen binding protein from Staphylococcus epidermidis was previously cloned, and the nucleotide sequence was determined. A portion of the gene encompassing the fibrinogen binding domain has now been subcloned in an expression-fusion vector. The fusion protein can bind to fibrinogen in a capture enzyme-linked immunosorbent assay and can be purified by fibrinogen affinity chromatography, This protein can completely inhibit the adherence of S. epidermidis to immobilized fibrinogen, suggesting that the adherence of S, epidermidis to fibrinogen is mainly due to this protein, Antibodies against this fibrinogen binding protein were also found to efficiently block the adherence of S. epidermidis to immobilized fibrinogen. Despite homology with clumping factors A and B from S. aureus (cell surface-associated proteins binding to fibrinogen), binding involved the beta chain of fibrinogen rather than the gamma chain, as in clumping factor A.

Publicerad i

Infection and Immunity
1999, Volym: 67, nummer: 9, sidor: 4525-4530
Utgivare: AMER SOC MICROBIOLOGY

SLU författare

• Guss, Bengt

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Mikrobiologi
Immunologi
Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1128/IAI.67.9.4525-4530.1999

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/79302