Sammanfattning

Programmed death and shedding of epithelial cells is a powerful defense mechanism to reduce bacterial burden during infection but this activity cannot be indiscriminate because of the critical barrier function of the epithelium. We report that during cystitis, shedding of infected bladder epithelial cells (BECs) was preceded by the recruitment of mast cells (MCs) directly underneath the superficial epithelium where they docked and extruded their granules. MCs were responding to interleukin-1 beta (IL-1 beta) secreted by BECs after inflammasome and caspase-1 signaling. Upon uptake of granule-associated chymase (mouse MC protease 4 [mMCPT4]), BECs underwent caspase-1-associated cytolysis and exfoliation. Thus, infected epithelial cells require a specific cue for cytolysis from recruited sentinel inflammatory cells before shedding.

Nyckelord

mast cell chymase; bladder exfoliation; apoptosis

Publicerad i

Immunity
2016, Volym: 45, nummer: 6, sidor: 1258-1269
Utgivare: CELL PRESS

SLU författare

• Åbrink, Magnus

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Immunologi inom det medicinska området


Publikationens identifierare

DOI: https://doi.org/10.1016/j.immuni.2016.11.003

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/80146